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Mastering HEK293 Cell Culture: A Beginner's Guide to Avoiding Common Pitfalls

Luoyang Fudau Biotech Co., Ltd.
Publiziert am 11. September 2025HEK293 cells are not "high-maintenance," but they demand "standardized operations." By selecting compliant consumables (e.g., sterile TC-treated flasks, low-endotoxin serum), controlling passage density and environmental parameters (temperature, CO₂, agitation speed), and implementing robust contamination prevention, beginners can quickly learn to reliably obtain high-viability HEK293 cells suitable for experiments like transfection, protein expression, and virus packaging.
