The cell culture flasks, also called the canted neck bottle, is a cell consumable suitable for medium-scale cell and tissue culture in the laboratory.
According to the different types of cultured cells, it is divided into TC hydrophilic surface (suitable for adherent cells) and non-TC hydrophobic surface (suitable for suspended cells). There are two types of bottle caps: seal cap and vent cap. The vent cap has a 0.2μm hydrophobic filter membrane, which provides sterile gas exchange and reduces the risk of contamination. It is mainly used for open culture and is widely used in cell culture technology centers.
Two kinds of bottle caps
Sealing cap: This kind of cap is completely sealed. There are no vents on the cap. It is mainly used under carbon dioxide-free conditions such as incubators and greenhouses. It has good airtightness and can prevent the invasion of external bacteria and promote cell reproduction. Create a good growth environment.
Breathable cover: This kind of cover is equipped with vent holes, these holes can let the carbon dioxide in the environment enter the cell culture square flask, and create suitable growth conditions for cell growth. There is a sterile breathable membrane on the top of the bottle cap, which has good waterproof and breathable functions. The liquid in the cell culture square bottle does not affect the microbial barrier and breathability of the breathable membrane after contact, which can ensure a good growth state of cells.