In biomedical research, the Erlenmeyer Shake Flask, with its unique conical design, serves as an ideal tool for cell culture. It not only provides cells with ample oxygen and nutrients but also effectively prevents contamination, ensuring a sterile cultivation environment. However, to fully leverage the advantages of the Erlenmeyer Shake Flask, the following key considerations must be observed during use:
1.Ensure Sterile Operation
Before using the Erlenmeyer Shake Flask, confirm that the flask is sterile. Even minor contamination can affect cell growth and lead to experimental failure. It is recommended to use sterilized products and perform cell inoculation in a biosafety cabinet.
2.Proper Culture Conditions
Place the Erlenmeyer Shake Flask in a constant-temperature shaker or incubator, and set appropriate temperature, humidity, and rotation speed. By adjusting the shaker's speed and amplitude, you can simulate the dynamic environment of cells in vivo, promoting uniform nutrient distribution and gas exchange. Regularly monitor cell growth and adjust culture conditions as needed.
3.Subculturing Procedure
When cell density reaches a certain level, subculturing is required. Handle cells gently during subculturing to avoid damage. Use an appropriate amount of trypsin or other dissociation reagents to ensure even detachment of cells from the flask wall.
4.Avoid Vigorous Shaking
Although the Erlenmeyer Shake Flask is suitable for use in shakers, excessive shaking may cause mechanical damage to cells, affecting their growth and proliferation. It is recommended to select an appropriate rotation speed (typically 80–120 rpm) based on the cell type and avoid sudden acceleration or deceleration.
5.Regular Medium Replacement
Replace the culture medium periodically and supplement it with fresh reagents according to cell growth rate and experimental requirements. Metabolic waste in old medium may inhibit cell growth, so timely replacement is crucial for maintaining cell health.
Why Choose the Erlenmeyer Shake Flask?
Efficient Oxygen Supply: The conical design enhances gas exchange, meeting cells' oxygen demands.
Contamination Prevention: The sealed structure effectively prevents external contaminants from entering.
Wide Applicability: Suitable for various cultivation methods, including suspension and adherent cell cultures.
The Erlenmeyer Shake Flask is an indispensable tool for cell culture, but its efficient use depends on proper operation and meticulous maintenance. By following the above guidelines, you can maximize the benefits of the Erlenmeyer Shake Flask and ensure smooth experiment progression.
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